Dr. Chambless received her medical degree from the Vanderbilt University School of Medicine and her bachelor of science in biological sciences from Stanford University in California.

Dr. Chambless completed her residency in Neurological Surgery and fellowship in Neurosurgical Oncology at Vanderbilt. She also completed a fellowship in Minimally Invasive Neurosurgical Oncology at the Centre for Minimally Invasive Neurosurgery in Sydney, Australia.